E5134

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

• Synonym(s): Sequestrene Na₂, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂
• Empirical Formula (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• CAS Number: 6381-92-6
• Molecular Weight: 372.24
• Beilstein: 3900609
• EC Number: 205-358-3
• UNSPSC Code: 12352107
• PubChem Substance ID: 24894605
• NACRES: NA.31

Properties

• grade: for molecular biology
• Assay: 99.0-101.0% (titration)
• form: powder
• reaction suitability: reagent type: chelator
• technique(s): electrophoresis: suitable
• impurities: ≤0.005% Insolubles; ≤0.1% Nitrilotriacetic acid (NTA)
• mp: 248 °C (dec.) (lit.)
• solubility: 3 M NaOH: 1.6 g/10 mL
• cation traces: Fe: ≤0.005%; Pb: ≤0.002%
• suitability: suitable for electrophoresis; suitable for molecular biology
• foreign activity: DNase, RNase, NICKase and protease, none detected
• storage temp.: room temp
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

General description

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

Application

EDTA disodium salt has been used in research:

• as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
• an anticoagulant for whole blood collection
• a stabilizer solution for freezing cell culture samples
• in combination with PBS and trypsin, to trypsinize cells
• for phytoextraction of heavy metals in polluted soils

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

• Molecular Biology grade, suitable for many molecular biology applications
• Free of endonucleases DNase, RNase, Nickase, and Protease

Other Notes

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Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H332 - H373
• Precautionary Statements: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves