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product name
IIC9 (CH0), 00030814
biological source
hamster embryo
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Chinese hamster embryo fibroblast
Cell Line Description
The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.
Application
IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.
Culture Medium
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin /EDTA; 37°C; 5% CO2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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