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Product Name
TM3, 91060526
biological source
mouse testis
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Prostaglandin F2a
receptors
Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse BALB/c testis, Leydig cell
Cell Line Description
Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.
Application
Hormone studies
Culture Medium
Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37²C.
Other Notes
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