Skip to Content
Merck
  • L-Ribulose production from L-arabinose by an L-arabinose isomerase mutant from Geobacillus thermodenitrificans.

L-Ribulose production from L-arabinose by an L-arabinose isomerase mutant from Geobacillus thermodenitrificans.

Biotechnology letters (2008-05-31)
Soo-Jin Yeom, Jung-Hwan Ji, Ran-Young Yoon, Deok-Kun Oh
ABSTRACT

Geobacillus thermodenitrificans, with a double-site mutation in L-arabinose isomerase, produced 95 g L-ribulose l(-1 ) from 500 g L-arabinose l(-1) under optimum conditions of pH 8, 70 degrees C, and 10 units enzyme ml(-1) with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75 degrees C were 35 and 4.5 h, respectively.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-Ribulose solution, ~1 M in H2O, ≥97.0% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
L-Ribulose, ≥90% (HPLC)
SKU
Pack Size
Availability
Price
Quantity