O9515
Prolyl oligopeptidase
recombinant, expressed in E. coli
Synonym(s):
Prolyl endopeptidase
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20 μG
HUF 205,000.00
HUF 205,000.00
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20 μG
HUF 205,000.00
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
HUF 205,000.00
Please contact Customer Service for Availability
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recombinant
expressed in E. coli
Quality Level
Assay
≥90% (SDS-PAGE)
form
solution
specific activity
≥10 unit/μg protein
mol wt
81.6 kDa
shipped in
dry ice
storage temp.
−70°C
General description
Prolyl oligopeptidase (PO) is mapped to human chromosome 6q22.[1] It comprises of a N-terminal β-propeller domain and C-terminal α/β hydrolase catalytic domain.[2] PO is highly expressed in the neuronal cytoplasm and its expression increases with age.[3]
Prolyl oligopeptidase is a cytosolic serine peptidase which cleaves peptide bonds at the C′ terminal side of prolines. It is only capable of processing peptides containing no more than 30 amino acids due to the unique β-propeller region that regulates access to the active site. [4]
Application
Biochem/physiol Actions
Prolyl oligopeptidase (PO) regulates physiological processes and is crucial for the generation of active hormone and peptide fragments from their precursors. It shows elevated levels in psychiatric disorders and Alzheimer′s patients.[8] Altered levels of PO is observed in patients with bipolar disorder.[1] High expression of PO may promote metastasis of malignant ovarian and colorectal tumors.[9]
Unit Definition
One unit will hydrolyze 1.0 picomole of Ala-Pro-aminomethylcoumarin per minute at pH 7.4 at 25 °C.
Physical form
Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT and variable amounts of imidazole.
Preparation Note
N-terminal GST-tagged 81.6 kDa full-length protein
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Structure-function properties of prolyl oligopeptidase family enzymes
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Cell Biochemistry and Biophysics, 44(3), 349-365 (2006)
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Distribution of Prolyl Oligopeptidase in Human Peripheral Tissues and Body Fluids
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Clinical Chemistry and Laboratory Medicine, 34, 17-22 null
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