NaHCO3: no phenol red: yes Earle’s salts (5% CO2): yes L-glutamine: no sodium pyruvate: no HEPES: no
shipped in
ambient
storage temp.
2-8°C
General description
Modification of the original MEM formulation. Contains a succinate buffering system. See formulations table for specific information. Medium may be autoclaved.
Other Notes
Modified for autoclaving.
Quantity
Formulated to contain 9.4 grams of powder per liter of medium.
Reconstitution
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.
Mammalian tissue- and/or time-specific transcription is primarily regulated in a combinatorial fashion through interactions between a specific set of transcriptional regulatory factors (TRFs) and their cognate cis-regulatory elements located in the regulatory regions. In exploring the DNA regions and TRFs
The axon initial segment (AIS) is critical for the initiation and propagation of action potentials. Assembly of the AIS requires interactions between scaffolding molecules and voltage-gated sodium channels, but the molecular mechanisms that stabilize the AIS are poorly understood. The
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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