F3290

FLAG^® Peptide

Name: FLAG<SUP>®</SUP> Peptide
Brand: SIGMA

≥85% (HPLC), lyophilized powder

Synonym(s):

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

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About This Item

Empirical Formula (Hill Notation):

C₄₁H₆₀N₁₀O₂₀

Molecular Weight:

1012.97

UNSPSC Code:

12352202

PubChem Substance ID:

329799698

NACRES:

NA.32

MDL number:

MFCD02262135

Key Documents

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Product Name

FLAG^® Peptide, lyophilized powder

SMILES string

NCCCC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](N)CC(O)=O)C(O)=O

InChI

1S/C41H60N10O20/c42-11-3-1-5-22(45-36(65)24(13-19-7-9-20(52)10-8-19)47-34(63)21(44)14-29(53)54)35(64)48-26(16-31(57)58)38(67)50-28(18-33(61)62)40(69)51-27(17-32(59)60)39(68)49-25(15-30(55)56)37(66)46-23(41(70)71)6-2-4-12-43/h7-10,21-28,52H,1-6,11-18,42-44H2,(H,45,65)(H,46,66)(H,47,63)(H,48,64)(H,49,68)(H,50,67)(H,51,69)(H,53,54)(H,55,56)(H,57,58)(H,59,60)(H,61,62)(H,70,71)/t21-,22-,23-,24-,25-,26-,27-,28-/m0/s1

InChI key

XZWYTXMRWQJBGX-VXBMVYAYSA-N

assay

≥85% (HPLC)

form

lyophilized powder

mol wt

1012.97 Da

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Related Categories

FLAG Purification

Protein Purification Chromatography

Protein Sample Prep

Recombinant/Fusion Tag Protein Purification

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Show Differences

1 of 4

This Item	F4799	SAE0194	I2149
Millipore F3290 FLAG^® Peptide	Sigma-Aldrich F4799 3X FLAG^® Peptide	Sigma-Aldrich SAE0194 Purified 3xFLAG^® peptide	Sigma-Aldrich I2149 Influenza Hemagglutinin (HA) Peptide
assay ≥85% (HPLC)	assay ≥90% (HPLC/MS)	assay ≥95% (HPLC)	assay ≥97% (HPLC)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form powder
mol wt 1012.97 Da	mol wt -	mol wt -	mol wt 1102.15 Da
shipped in wet ice	shipped in wet ice	shipped in dry ice	shipped in -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C

Application

Gently elutes FLAG fusion proteins from ANTI-FLAG^® M1 and M2 affinity resins. Usual working concentration is 100 μg/ml. Will not elute 3X FLAG fusion proteins. For this application, use 3X FLAG Peptide, F4799.

Learn more product details in our FLAG^® application portal.

General description

The FLAG peptide is used for the competitive elution of amino-terminal, Met-amino-terminal or carboxy-terminal FLAG fusion proteins from the Anti-FLAG M1 or Anti-FLAG M2 antibody (in solution or bound to an agarose gel).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Preparation Note

For stock solution, dissolve in TBS (10mM Tris HCL, 150 mM NaCl, pH7.4) to a final concentration of 5 mg/mL.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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A membrane protein preserves intrabacterial pH in intraphagosomal Mycobacterium tuberculosis.

Omar H Vandal et al.

Nature medicine, 14(8), 849-854 (2008-07-22)

Acidification of the phagosome is considered to be a major mechanism used by macrophages against bacteria, including Mycobacterium tuberculosis (Mtb). Mtb blocks phagosome acidification, but interferon-gamma (IFN-gamma) restores acidification and confers antimycobacterial activity. Nonetheless, it remains unclear whether acid kills

Deficiency in 3'-phosphoglycolate processing in human cells with a hereditary mutation in tyrosyl-DNA phosphodiesterase (TDP1).

Tong Zhou et al.

Nucleic acids research, 33(1), 289-297 (2005-01-14)

Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that removes peptide fragments linked through tyrosine to the 3' end of DNA, and can also remove 3'-phosphoglycolates (PGs) formed by free radical-mediated DNA cleavage. To assess whether TDP1 is primarily responsible

The detection limit of a Gd3+-based T1 agent is substantially reduced when targeted to a protein microdomain.

Kenjiro Hanaoka et al.

Magnetic resonance imaging, 26(5), 608-617 (2008-02-01)

Simple low molecular weight (MW) chelates of Gd(3+) such as those currently used in clinical MRI are considered too insensitive for most molecular imaging applications. Here, we evaluated the detection limit (DL) of a molecularly targeted low MW Gd(3+)-based T(1)

Assembly of Drosophila centromeric chromatin proteins during mitosis.

Barbara G Mellone et al.

PLoS genetics, 7(5), e1002068-e1002068 (2011-05-19)

Semi-conservative segregation of nucleosomes to sister chromatids during DNA replication creates gaps that must be filled by new nucleosome assembly. We analyzed the cell-cycle timing of centromeric chromatin assembly in Drosophila, which contains the H3 variant CID (CENP-A in humans)

Signaling events downstream of mammalian target of rapamycin complex 2 are attenuated in cells and tumors deficient for the tuberous sclerosis complex tumor suppressors.

Jingxiang Huang et al.

Cancer research, 69(15), 6107-6114 (2009-07-16)

Mutations in the TSC1 and TSC2 tumor suppressor genes give rise to the neoplastic disorders tuberous sclerosis complex (TSC) and lymphangioleiomyomatosis. Their gene products form a complex that is a critical negative regulator of mammalian target of rapamycin (mTOR) complex

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FLAG® Peptide