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I6505

Sigma-Aldrich

Diethylaminoethyl–Sephacel®

aqueous ethanol suspension, 40-160 μm (wet), exclusion limit ~1,000,000 Da

Synonym(s):

DEAE-Sephacel®

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About This Item

CAS Number:
9013-34-7
MDL number:
MFCD00146622
UNSPSC Code:
47101511
PubChem Substance ID:
24896090
NACRES:
NA.56

form

aqueous ethanol suspension

technique(s)

activity assay: suitable

particle size

40-160 μm (wet)

pore size

~1,000,000 Da exclusion limit

pH

2—12

capacity

100-140 μeq/mL, gel

storage temp.

2-8°C

SMILES string

OC[C@@H]1O[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](O)O[C@H]2CO)[C@@H](O)[C@H](O)[C@H]1O

InChI

1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1

InChI key

GUBGYTABKSRVRQ-WFVLMXAXSA-N

General description

I6505-500ML′s update product number is GE17-0500-01

Application

Diethylaminoethyl (DEAE) Sephacel® is used in protein chromatography, ion exchange chromatography, and anion exchange media. It has been used to study sperm motility, sperm capacitation and sperm-oocyte interactions. DEAE Sephacel has also been used to study patients with immunoglobulin A (IgA) nephropathy. Diethylaminoethyl–Sephacel® has been used:

  • in the purification of media samples by ion-exchange chromatography
  • in the isolation of glycosaminoglycans (GAG) from cultured animal cells
  • in the purification of cytotoxic fractions from Tritrichomonas foetus isolates

Physical form

Suspension in 20% ethanol

Legal Information

Sephacel is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

129.2 °F

Flash Point(C)

54 °C

Certificates of Analysis (COA)

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Pierina De Muro et al.
Scandinavian journal of urology and nephrology, 41(3), 230-236 (2007-05-01)
A 2-year follow-up study was carried out in patients with IgA nephropathy (IgAN) in order to verify the possible use of quali-quantitative analysis of urinary glycosaminoglycans (GAGs) as a prognostic index of disease and for drug treatment monitoring. Ten patients
Daniel R Studelska et al.
Glycobiology, 16(1), 65-72 (2005-09-17)
Glycosaminoglycans (GAGs) are linear polysaccharides made by all animal cells. GAGs bind to hundreds of proteins, such as growth factors, cytokines, chemokines, extracellular matrix components, protease inhibitors, proteases, and lipoprotein lipase, through carbohydrate and protein interactions. These interactions control many
A Ortiz et al.
Nucleic acids research, 24(16), 3280-3281 (1996-08-15)
A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or
Yuan-Hong Wang et al.
Acta biochimica et biophysica Sinica, 38(9), 633-638 (2006-09-06)
Extracellular alginate lyase secreted by marine Vibrio sp. YWA, isolated from decayed Laminaria japonica, was purified by a combination of ammonium sulfate precipitation and diethylaminoethyl-Sephacel column chromatography. The results show that the molecular mass of alginate lyase was approximately 62.5
V T D'Souza et al.
Biochemical and biophysical research communications, 129(3), 727-732 (1985-06-28)
An artificial chymotrypsin, with all the features of the real chymotrypsin, namely a binding site (from cyclodextrin) attached to a catalytic site containing an imidazolyl group, a carboxylate group and a hydroxyl group, has been synthesized. This artificial chymotrypsin has
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