DFF100

DEAE–Sepharose^™

Fast Flow

• Synonym(s): Diethylaminoethyl–Sepharose^™
• CAS Number: 57407-08-6
• UNSPSC Code: 47101511
• NACRES: NA.56
• MDL number: MFCD00146630

Properties

• Quality Segment: 200
• form: suspension
• technique(s): LPLC: suitable, affinity chromatography: suitable
• matrix: 6% cross-linked agarose
• bead size: 45-165 μm (wet)
• pore size: ~4,000,000 Da exclusion limit
• pH: 2—12
• capacity: 110-160 μeq/mL binding capacity(gel volume)

Description

General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose has been used in:

• anion exchange chromatography
• to screen polyisoprenyl phosphate phosphatase activity
• to purify isoinhibitors
• for the purification of human immunodeficiency virus (HIV)
• glycoprotein envelope (gp140 env)

DEAE-Sepharose^™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose^™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Codes: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• Flash Point (°F): 100.4 - 109.4 °F
• Flash Point (°C): 38 - 43 °C
• PPE (personal protective equipment): Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter