A7094

L-Asparagine monohydrate

≥98.0%, suitable for cell culture, BioReagent, non-animal source

• Synonym(s): Asparagine monohydrate, (S)-(+)-2-Aminosuccinamic acid, (S)-2-Aminosuccinic acid 4-amide, L-Aspartic acid 4-amide
• Linear Formula: NH₂COCH₂CH(NH₂)COOH · H₂O
• CAS Number: 5794-13-8
• Molecular Weight: 150.13
• Beilstein: 1723527
• EC Number: 200-735-9
• MDL number: MFCD00151038
• UNSPSC Code: 12352209
• PubChem Substance ID: 24891175
• NACRES: NA.26

Properties

• Product Name: L-Asparagine monohydrate, from non-animal source, BioReagent, suitable for cell culture, ≥98.0%
• biological source: non-animal source
• Quality Level: 300
• product line: BioReagent
• Assay: ≥98.0%
• form: powder
• optical activity: [α]20/D 33.0 to 36.3 °, c = 10 in 6 M HCl
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: white
• mp: 233-235 °C (lit.)
• solubility: H₂O: 20 mg/mL
• cation traces: Fe: ≤10 ppm; heavy metals (as Pb): ≤10 ppm; <0.1% (Ammonia (NH3))
• functional group: amide; amine; carboxylic acid
• storage temp.: room temp
• SMILES string: [H]O[H].N[C@@H](CC(N)=O)C(O)=O
• InChI: 1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1
• InChI key: RBMGJIZCEWRQES-DKWTVANSSA-N

Description

General description

L-Asparagine is a naturally occurring amino acid found in various proteins. It is used in laboratory research for various applications, including cell culture, protein biochemistry, and nitrogen metabolism studies. Its solubility in water and role as a source of asparagine make it a versatile tool for investigating biological processes and chemical synthesis. Further, L-Asparagine residues in proteins can be crucial for protein stability, folding, and interactions.

Application

L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination in Bacillus subtilis has been increased in the presence of L-asparagine. L-Asparagine, a proteinogenic amino acid, from non-animal source is used in biomanufacturing cell culture systems for the production of therapeutic recombinant proteins and monoclonal antibodies. It has also been used as a component of LHC basal media for the culture of human airway epithelial cells.[1]

Biochem/physiol Actions

The amino acid L-asparagine is a structural analog of L-aspartic acid, where the side chain of the carboxylic acid moiety is amidated to give a terminal amine group.[2] This renders L-asparagine neutral at physiological pH. The amide group of asparagine is derived from glutamine,[3] in the reaction of aspartate and glutamine in the presence of ATP to yield asparagine and glutamate. In vivo, asparagine is hydrolyzed to aspartate and NH4⁺ by asparaginase.[4] Asparagine is also an important amino acid in glycopeptide bonds, via N-glycosyl linkages to the sugar rings.

Features and Benefits

• Suitable for Cell Culture and Biochemical research
• High-quality compound suitable for multiple research applications

Other Notes

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Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves