Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

G005622

GC Analysis of FAMEs in Cookies on SLB®-IL111 after SPE Fractionation using Discovery® Ag-Ion

suitable for GC, application for SPE

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

technique(s)

gas chromatography (GC): suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: 1 g of commercially purchased cookies was ground and subjected to acid digestion and alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09
SPE tube/cartridge: Discovery Ag-Ion SPE tubes, 750 mg/6 mL (54225-U)
condition: 4 mL of acetone; allow solvent to gravity drip completely through tube; discard eluant; 4 mL of hexane; allow solvent to gravity drip completely through tube; discard eluant
sample addition: 1 mL of extract; discard any eluant that drips through tube
elution: (Fraction 1) 6 mL of hexane:acetone (96:4); collect eluant in a fresh container with slight vacuum; ((Fraction 2) 4 mL of hexane: acetone (90:10); collect eluant in a fresh container with slight vacuum; (Fraction 3) 4 mL of 100% acetone; collect eluant in a fresh container with slight vacuum)
eluate post-treatment: evaporate each fraction at room temperature using nitrogen; reconstitute each fraction to 1 mL of hexane
column: SLB-IL111, 100 m x 0.25 mm I.D., 0.20 μm (29647-U)
oven: 168 °C
inj. temp.: 250 °C
detector: FID, 250 °C
carrier gas: 1 μL, 10:1 split
injection: 1 μL, 10:1 split
liner: 4 mm I.D., split type, single taper wool packed FocusLiner design

suitability

application for SPE

application(s)

food and beverages

Analysis Note

A sample of commercially purchased cookies was ground, and fatty acids were extracted, methylated, fractionated, and analyzed with GC-FID using the SLB-IL111. The nutritional label for the cookies included partially hydrogenated cottonseed and soybean oils as ingredients. Based on this information, the fatty acid profile obtained for this sample was as expected; with cottonseed oil accounting for the small amount of C14:0 FAME, and the soybean oil as the source of the small amount of C22:0 FAME detected. Both oils contributed to the higher levels of the C16:0, C18:0 and C18:1 FAMEs. Note the presence of the C18:1 trans isomers in fraction 1. These are the result of the partial hydrogenation of these oils.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany

Analytical column

Referencia del producto
Descripción
Precios

Related product

Referencia del producto
Descripción
Precios

SPE tube or plate

Referencia del producto
Descripción
Precios

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

Lo sentimos, en este momento no disponemos de COAs para este producto en línea.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico