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53826-U

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Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

Sinónimos:

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

Columna para HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

Materiales

stainless steel column

Nivel de calidad

100

Agency

suitable for USP L1

Línea del producto

Ascentis®

Características

endcapped

fabricante / nombre comercial

Ascentis®

envase

1 ea of

Parámetros

60 °C temp. range
600 bar max. pressure (9000 psi)

técnicas

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × D.I.

5 cm × 4.6 mm

superficie

135 m2/g

impurezas

<5 ppm metals

Matriz

Fused-Core particle platform
superficially porous particle

grupo activo de la matriz

C18 (octadecyl) phase

tamaño de partícula

2.7 μm

tamaño de poro

90 Å

pH operativo

2-9

aplicaciones

food and beverages

técnica de separación

reversed phase

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Descripción general

Las columnas de HPLC Ascentis Express, a través del uso de tecnología de partículas Fused-Core®, pueden proporcionarle la elevada velocidad y las elevadas eficiencias de las partículas inferiores a 2 μm a la vez que mantienen menores contrapresiones. La combinación de gran eficiencia y poca contrapresión beneficia a los usuarios de UPLC® (u otro sistema de presión ultraelevada), así como a los usuarios de la HPLC convencional.
Si desea más información sobre esta nueva tecnología de columna, visite la página de inicio Ascentis Express.

Aplicación

Análisis mediante HPLC de las capsaicinas en chile en polvo en Ascentis® Express C18, 2,7 μm
Análisis mediante UHPLC/MS de aceite de maíz en Ascentis® Express C30 en comparación con Ascentis® Express C18
Análisis mediante UHPLC del paracetamol e impurezas en Ascentis® Express C18, 2,7 μm
Análisis mediante HPLC de los parabenos en Ascentis®Express C18, 2,7 μm

Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

Aplicación

Referencia del producto
Descripción
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cartucho de precolumna

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Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317

Consejos de prudencia

P280

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
USUT007344
USUT007343
USUT007342
USUT007341
USUT007340

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Phytochemical Characterization of Rhus coriaria L. Extracts by Headspace Solid-Phase Micro Extraction Gas Chromatography, Comprehensive Two-Dimensional Liquid Chromatography, and Antioxidant Activity Evaluation
Arena K, et al.
Molecules (Basel), 27(5), 1727-1727 (2022)
Stefany Grutzmann Arcari et al.
Electrophoresis, 41(20), 1784-1792 (2020-08-12)
This study aimed to evaluate the polyphenolic composition along with the biological activity of guabiroba (Campomanesia xanthocarpa Berg.) fruits using comprehensive two-dimensional liquid chromatography (LC × LC). A simplex centroid design comprising three solvents (methanol, 2% acetic acid, and acetonitrile) was used
Katia Arena et al.
Analytical and bioanalytical chemistry, 415(13), 2371-2382 (2022-07-15)
In this work, a comprehensive two-dimensional liquid chromatography system, comprised of a ZIC-HILIC and C18 columns in the first and second dimensions, respectively, was tuned and employed for attaining high resolution profiles of the polyphenolic pattern in seven commercial berry
Adriana Arigò et al.
Journal of chromatography. A, 1649, 462183-462183 (2021-05-27)
In the European Union, cosmetic products are regulated by the Cosmetics Regulation (EC) No. 1223/2009. The Analytical Working Group of the International Fragrance Association (IFRA) suggested that a new sensitive analytical method is needed to determine psoralens in finished cosmetic
Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
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Artículos

Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC

Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.

Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC

Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.

Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC

Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.

Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC

Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.

Ver todo

Chromatograms

application for HPLC, application for SPEapplication for HPLCapplication for HPLCapplication for HPLC, application for SPE

Questions

1–9 of 9 Questions  

  1. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 answer

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Helpful?

  2. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 answer

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 answer

    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      Helpful?

  5. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 answer

    1. Nothing special is required to use Ascentis Express HPLC columns.  To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.  For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).

      Helpful?

  6. Can I use Ascentis Express on a UHPLC system?

    1 answer

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  7. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 answer

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      Helpful?

  8. Can I use Ascentis Express on any type of HPLC system?

    1 answer

    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      Helpful?

  9. What is the carbon load for Ascentis® Express C18 HPLC Columns?

    1 answer

    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared

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