X4626

XTT sodium salt

powder, BioReagent, suitable for cell culture

• Sinónimos: 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
• Fórmula empírica (notación de Hill): C₂₂H₁₆N₇NaO₁₃S₂
• Número de CAS: 111072-31-2
• Peso molecular: 673.52
• Número MDL: MFCD00083517
• Código UNSPSC: 12352207
• ID de la sustancia en PubChem: 24902150
• NACRES: NA.75

Propiedades

• Línea del producto: BioReagent
• Nivel de calidad: 200
• Ensayo: ≥90%
• Formulario: powder
• condiciones de almacenamiento: dry at room temperature
• técnicas: cell culture | mammalian: suitable; protein quantification: suitable
• aplicaciones: detection
• método de detección: colorimetric
• temp. de almacenamiento: 2-8°C
• cadena SMILES: [Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O
• InChI: 1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1
• Clave InChI: JACYMBNQPPWQML-UHFFFAOYSA-M

Descripción

Descripción general

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Aplicación

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

XTT sodium salt has been used to assess cell viability.

Acciones bioquímicas o fisiológicas

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

Principio

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Danger
• Frases de peligro: H242
• Consejos de prudencia: P210 - P234 - P235 - P240 - P370 + P378 - P403
• Clasificaciones de peligro: Self-react. C
• Riesgos supl.: EUH044
• Código de clase de almacenamiento: 4.1A - Other explosive hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)