T3809

Reactivo TRI^®

BD, For processing whole blood, plasma, or serum.

• Sinónimos: Reactivo para aislamiento de ARN TRI Reagent^®
• MDL number: MFCD00213058
• UNSPSC Code: 12352200
• NACRES: NA.52

Propiedades

• Quality Level: 200
• usage: 0.75 mL sufficient for 0.25 mL blood derivatives

Descripción

General description

TRI Reagent BD is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein from serum, plasma or whole blood. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA and protein. This procedure is an adaptation of the single-step method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of blood derivatives.

TRI REAGENT BD performs well with large or small sample volumes, and many samples can be simultaneously extracted. TRI Reagent BD is a mixture of guanidine thiocyanate and phenol in a mono-phase solution. When a sample of blood derivatives is lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI REAGENT BD processes 0.25 ml of blood derivatives.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh cells. The procedure is very effective for isolating RNA molecules of all types from 0.1 to 15 kb in length. The resulting RNA is intact with little or no contaminating DNA or protein.

TRI Reagent^® is a monophasic solution of phenol and guanidinium isothiocyanate.

Application

TRI Reagent^® has been used for the isolation of RNA.

The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assays, cloning and PCR.

El reactivo TRI es una versión mejorada del reactivo de aislamiento del ARN total en una etapa desarrollado por Chomczynski. El método de aislamiento del ARN basado en este reactivo es ampliamente utilizado y probado para aplicaciones del ARN. Es ideal para el aislamiento rápido, económico y eficiente del ARN total o el aislamiento simultáneo del ARN, el ADN y las proteínas de muestras de origen humano, animal, vegetal, de levaduras, bacteriano y vírico.

Features and Benefits

• Aislamiento del ARN fácilmente escalable
• Funciona con muchos orígenes: humano, vegetal, levaduras, bacterias o vírico
• Mejor rendimiento que los métodos tradicionales de tiocianato de guanidina/cloruro de cesio

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Información de seguridad

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H311 + H331,H314,H341,H373,H411
• pcodes: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• target_organs: Nervous system,Kidney,Liver,Skin
• supp_hazards: EUH032
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3
• flash_point_f: 174.2 °F - closed cup
• flash_point_c: 79 °C - closed cup