MRN10
GenElute™ mRNA Miniprep Kit
sufficient for 10 purifications
Sinónimos:
GenElute™ mRNA Kit, Gen Elute
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
41105501
NACRES:
NA.52
Productos recomendados
uso
sufficient for 10 purifications
Nivel de calidad
técnicas
RNA purification: suitable
temp. de almacenamiento
15-25°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Categorías relacionadas
Descripción general
Procedures such as cDNA synthesis, expression profiling and others require separation of mRNA from the vastly more abundant rRNA and tRNA. The GenElute mRNA kits provide convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues. For direct mRNA preparation, cells or tissues are disrupted with SDS/proteinase K digestion to release RNA and eliminate RNases. Both kit types use oligo dT30 covalently linked to 1 μm polystyrene beads to capture polyadenylated mRNA by hybridization. The polystyrene beads remain suspended during hybridization, eliminating the need for mixing or rocking, as is common for cellulose or magnetic particles. Polystyrene was also chosen because oligo(dT) polystyrene beads yield cleaner mRNA with fewer stringent washing steps than does the more commonly used oligo(dT) cellulose (2 or 3 wash steps versus 10 or more). With the GenElute kits, mRNA-bead complexes are washed on a microcentrifuge spin filter, and eluted into 10 mM Tris-HCL, pH 7.5. mRNA prepared with either kit is suitable for a variety of downstream applications such as Northern blotting, expression array or chip hybridizations and cDNA synthesis and library construction.
The GenElute mRNA Miniprep Kit provides a simple and convenient way to purify polyadenylated mRNA from previously isolated total RNA. Oligo(dT) polystyrene beads bind the poly(A)+ mRNA during a 10 minute incubation. After washing in a microspin filter to remove contaminants, the poly(A)+ mRNA is eluted in 100 mL of buffer. Purification of mRNA from total RNA, can be performed in less than 40 minutes.
The purified mRNA is ready for Northern analysis, reverse transcription and PCR, labeling for arrays, and other common applications.
The purified mRNA is ready for Northern analysis, reverse transcription and PCR, labeling for arrays, and other common applications.
Aplicación
The purified mRNA is ready for Northern analysis, reverse transcription and PCR, labeling for arrays, and other common applications.
Características y beneficios
- Quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein
- Performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted
- One of the most effective methods for isolating total RNA. Purifications can be completed in only one hour starting with fresh tissue or cells
- mRNA captured on oligo(dT) polystyrene beads in 10 minutes
- Oligo(dT) polystyrene beads require fewer wash steps
- Poly (A)+ mRNA isolated from previously purified total RNA in 40 minutes
Otras notas
For additional information, please see www.sigma-aldrich.com/mrna.
Información legal
GenElute is a trademark of Sigma-Aldrich Co. LLC
Código de clase de almacenamiento
10 - Combustible liquids
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Jianzi Lan et al.
Cellular & molecular biology letters, 27(1), 51-51 (2022-06-28)
Diabetic nephropathy (DN) is prevalent in patients with diabetes. N6-methyladenosine (m6A) methylation has been found to cause modification of nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing (NLRP) 3, which is involved in cell pyroptosis and inflammation. WTAP is a
Gang Luo et al.
Foods (Basel, Switzerland), 11(11) (2022-06-11)
N6-methyladenosine (m6A) is the most prevalent internal mRNA modification in eukaryotes. The M6A modification plays an important role in transcription and cell function. The mechanism by which m6A modification regulates meat quality remains elusive. In this study, gene knockout and
Zhihui Xu et al.
Frontiers in plant science, 12, 685189-685189 (2021-06-29)
The remodeling of transcriptome, epigenome, proteome, and metabolome in hybrids plays an important role in heterosis. N(6)-methyladenosine (m6A) methylation is the most abundant type of post-transcriptional modification for mRNAs, but the pattern of inheritance from parents to hybrids and potential
Caitlin M A Simopoulos et al.
G3 (Bethesda, Md.), 9(8), 2511-2520 (2019-06-27)
Long non-coding RNAs (lncRNAs) represent a diverse class of regulatory loci with roles in development and stress responses throughout all kingdoms of life. LncRNAs, however, remain under-studied in plants compared to animal systems. To address this deficiency, we applied a
Liping Wu et al.
Frontiers in immunology, 13, 839677-839677 (2022-06-28)
Host translation is generally modulated by viral infection, including duck hepatitis A virus (DHAV) infection. Previously, we reported that cellular protein synthesis in a cell model of duck embryo fibroblasts is significantly inhibited by DHAV infection but not viral proteins
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico