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Key Documents

SRP3269

Sigma-Aldrich

RANTES human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Sinónimos:

CCL5, SIS-delta

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-10.0 ng/mL

mol wt

7.8 kDa

packaging

pkg of 20 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CCL5(6352)

General description

Regulated on activation, normal T cell expressed and secreted (RANTES) also known as C-C motif chemokine ligand 5 (CCL5) is encoded by the gene mapped to human chromosome 17. RANTES belongs to intercrine or chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections. Recombinant human RANTES is a 7.8kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Application

RANTES human has been used in following studies:
  • In transmigration experiments to determine whether LFA-1 cross-linking could affect peripheral blood T lymphocytes (PBTL) migration in response to the chemokine RANTES.
  • Luminol-dependent chemiluminescence assay to determine the effect of RANTES on reactive oxygen species (ROS) production by eosinophils.

Biochem/physiol Actions

Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4+/CD45RO), basophils, and eosinophils. The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5.1 RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. The In1.1C variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV-1 infection.

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Inside the crawling T cell: leukocyte function-associated antigen-1 cross-linking is associated with microtubule-directed translocation of protein kinase C isoenzymes beta(I) and delta.
Volkov Y
Journal of Immunology, 161(12), 6487-6495 (1998)
The Role of Genetic Polymorphisms in the
Chemokine and Their Receptors and Cytokines
in the Human Immunodeficiency Virus Type 1
(HIV-1) Infection
Nancy Dumais
HIV and AIDS - Updates on Biology, Immunology, Epidemiology and Treatment Strategies (2011)
A role for the chemokine RANTES in regulating CD8 T cell responses during chronic viral infection.
Crawford A
PLoS Pathogens, 7(7), e1002098-e1002098 (2011)
RANTES-activated human T lymphocytes. A role for phosphoinositide 3-kinase.
Turner L
Journal of Immunology, 155(5), 2437-2444 (1995)
J J Oppenheim et al.
Annual review of immunology, 9, 617-648 (1991-01-01)
A family consisting of at least ten distinct novel 8-10 kd cytokines has been identified over the past 12 years. These cytokines exhibit from 20 to 45% homology in amino acid sequence, are probably all basic heparin-binding polypeptides, and have

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