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SMB00610

Sigma-Aldrich

Lipopolysaccharide from Porphyromonas gingivalis

purified by phenol extraction

Sinónimos:

LPS

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About This Item

Código UNSPSC:
12352211
NACRES:
NA.25

origen biológico

Porphyromonas gingivalis

Nivel de calidad

Formulario

powder

purificado por

phenol extraction

impurezas

≤3% Protein (Lowry)

color

white to faint yellow

solubilidad

triethylene glycol dimethyl ether: 0.90-1.10 mg/mL to hazy, colorless to light yellow

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

Descripción general

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria like Porphyromonas gingivalis. It differs from LPS from Escherichia coli in its structure and various functional activities.

Aplicación

Lipopolysaccharide has been used:

  • as a treatment to stimulate acute lung injury in human bronchial epithelial (HBE) cells
  • as an agonist for toll-like receptor 4 (TLR4) and to stimulate intestinal normal fibroblasts (NFs) to study osteopontin (OPN) expression in myofibroblasts
  • to study its effects on pro-inflammatory and pro-coagulant genes expression in endothelial cells

Acciones bioquímicas o fisiológicas

LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular patterns (PAMPs).

Additionally, it was demonstrated that the mechanisms by which LPS from E. coli and P. gingivalis modulate cluster of differentiation 14 (CD14), toll-like receptor 2 (TLR2), and toll-like receptor 4 (TLR4) surface expression, primary and secondary cytokine responses are different.

Porphyromonas gingivalis is a Gram-negative bacterium that is known to be involved in adult periodontitis. Periodontitis is a chronic inflammatory disease characterized by the recession of the supportive tissue surrounding teeth. Studies have shown that the LPS from P. gingivalis plays an important role in this disease.
A recent study demonstrated that LPS from P. gingivalis stimulates insulin secretion by the pancreatic β cell line, MIN6. In the presence of 5 mM glucose and 50-500 ng/mL LPS from P. gingivalis, a significant induction of insulin secretion was observed.

Nota de preparación

LPS from P. gingivalis is soluble in water and cell culture medium DMEM (1-5 mg/mL), yielding a clear solution.

Otras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Epidemiology of subgingival bacterial pathogens in periodontal disease
Zambon, J. J., et al.
Molecular Pathogenesis of Periodontal Disease, 1, 1-12 (1994)
Diya Zhang et al.
Innate immunity, 14(2), 99-107 (2008-08-21)
Lipopolysaccharide (LPS) derived from the periodontal pathogen Porphyromonas gingivalis has been shown to differ from enterobacterial LPS in structure and function; therefore, the Toll-like receptors (TLRs) and the intracellular inflammatory signaling pathways are accordingly different. To elucidate the signal transduction
Analysis of Lipopolysaccharides of Gram-Negative Bacteria
Mayer, H. et al.
Methods in Microbiology, 18, 157-207 (1985)
H E Barksby et al.
Clinical and experimental immunology, 156(3), 479-487 (2009-05-15)
Porphyromonas gingivalis lipopolysaccharide (LPS) (strain W50) interacts with Toll-like receptor 2 (TLR-2) leading to cytokine expression and inflammation, and thereby plays a key role in the pathogenesis of periodontal disease. The aims of this study were to investigate gene expression
Epidemiology of subgingival bacterial pathogens in periodontal disease.
Zambon, J.J., et al.
Molecular Pathogenesis of Periodontal Disease, 1-12 (1994)

Artículos

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

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