L2880

Lipopolysaccharides from Escherichia coli O55:B5

purified by phenol extraction

• Sinónimos: LPS
• Número de CAS: 93572-42-0
• Número CE: 297-473-0
• Número MDL: MFCD00164401
• Código UNSPSC: 12352201
• NACRES: NA.25

Propiedades

• origen biológico: Escherichia coli (O55:B5)
• Nivel de calidad: 300
• Formulario: lyophilized powder
• purificado por: phenol extraction
• técnicas: cell based assay: suitable
• impurezas: <3% Protein (Lowry)
• color: off-white to tan
• solubilidad: water: soluble
• aplicaciones: cell analysis; clinical research; life science and biopharma
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Lipopolysaccharides (LPS) are glycolipids, a combination of carbohydrate units linked with single or multiple fatty acids that are found in the cell wall of Gram-negative bacteria. The LPS is an essential component of the outer membrane and the LPS structure is composed of lipid A, a glucosamine-based phospholipid, a short-core oligosaccharide, and an O-antigen, a distal polysaccharide.

Aplicación

Lipopolysaccharide from Escherichia coli O55:B5 has been used:

• to stimulate hepatocytes and non-parenchymal cells
• to stimulate the spleen and study the expression of the Malitaf gene in the fish blunt snout bream
• as part of the concanavalin A (ConA) and D-galactosamine/lipopolysaccharide (D-GalN/LPS) model of fulminant hepatitis to study the effect of the natural compound hesperetin
• to check the cell viability of human chorionic villi-derived mesenchymal stem cells (hCMSCs) by methyl thiazolyl tetrazolium (MTT) assay
• to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of few compounds
• to induce sarcopenia in rats to evaluate the antioxidant effects of curcumin-loaded hydrophobic surface-modified hydroxyapatite

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Acciones bioquímicas o fisiológicas

Lipopolysaccharides (LPS) sets up a protective permeability barrier that blocks the entry of antibiotics and bile salts, which make Gram-negative bacteria innately resistant. LPS is involved in bacterial pathogenicity as the host immune system primarily encounters the LPS component of the bacteria. LPS is an endotoxin and a highly immunogenic antigen that can enhance the immune system. The lipid A of LPS stimulates host cells via toll-like receptors 4 (TLR4).

Otras notas

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For optimal reconstitution of LPS products, an intensive vortex is required before use, regardless of the container type. Ensure that the storage solution concentration is at least 1 mg/ml. While borosilicate (silanized) containers are recommended, polypropylene (PP) plastic containers may also be used.

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Información de seguridad

• Pictogramas: GHS06
• Palabra de señalización: Danger
• Frases de peligro: H300
• Consejos de prudencia: P264 - P270 - P301 + P310 - P405 - P501
• Clasificaciones de peligro: Acute Tox. 2 Oral
• Código de clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)