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Merck

RAB0228

Sigma-Aldrich

Human IGF-I ELISA Kit

for serum, plasma, cell culture supernatant and urine

Sinónimos:

Insulin-like growth factor I, Mechano growth factor, Somatomedin-C

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.123-30 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IGF1(3479)

General description

The Human IGF-I ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF-I in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human IGF-I

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials


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Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

Various growth factor yields in various platelet rich plasma processing methods.
Sari P
BioTechnology: An Indian Journal, 9 (2014)
Effects of insulin and insulin-like growth factors on cultured human hair follicles: IGF-I at physiologic concentrations is an important regulator of hair follicle growth in vitro.
Philpott MP, et. al.
The Journal of Investigative Dermatology, 102, 857-857 (1994)
Activation of the IGF1 pathway mediates changes in cellular contractility and motility in single-suture craniosynostosis.
Al-Rekabi Z
Journal of Cell Science, 129 (2016)
A Blutke et al.
Molecular and cellular endocrinology, 394(1-2), 88-98 (2014-07-16)
To study insulin-like growth factor 1 (IGF1)-independent effects of permanent growth hormone (GH) excess on body and organ growth and pathology in vivo, hemizygous bovine GH transgenic mice with homozygous disruption of the Igf1 gene (Igf1(-/-)/GH) were generated, and examined
Effects of insulin and insulin-like growth factors on cultured human hair follicles: IGF-I at physiologic concentrations is an important regulator of hair follicle growth in vitro.
Philpott MP
The Journal of Investigative Dermatology, 102, 857-861 (1994)

Questions

  1. Does this kit detect only the free form of IGF-1, or does it also detect IGF-1 bound to binding proteins?

    1 answer
    1. The standard utilized in this kit is recombinant human IGF-1, derived from E. coli and corresponds to amino acids 49-118 of the human IGF-I protein (P05019).
      Specificity: This antibody pair is designed to detect only the free form of human IGF-1 and does not recognize IGF-1 when it is complexed with IGF binding proteins (IGFBPs). Furthermore, the IGF-1 ELISA kit has been tested and shows no cross-reactivity with a variety of other cytokines, including Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1 through IGFBP-4, IL-1 alpha through IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, and VEGF.

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