P5994

Sustrato de fosfatasa

40 mg tablets

• Sinónimos: Fosfato de 4-nitrofenilo disodium salt hexahydrate, pNPP disodium salt hexahydrate
• Fórmula lineal: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• Número de CAS: 333338-18-4
• Peso molecular: 371.14
• Número CE: 224-246-5
• Número MDL: MFCD00066288
• Código UNSPSC: 12352204
• ID de la sustancia en PubChem: 24898723
• NACRES: NA.54

Propiedades

• Nivel de calidad: 200
• Formulario: tablet
• concentración: 36-44 mg/tablet
• impurezas: p-nitrophenol, essentially free
• solubilidad: H₂O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
• temp. de almacenamiento: −20°C
• cadena SMILES: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• Clave InChI: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Descripción

Descripción general

Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.

Aplicación

Sustrato cromógeno para usar en procedimientos ELISA de fosfatasa ácida y la alcalina. Rinde un producto final soluble que es de color amarillo y puede leerse espectrofotométricamente a 405 nm. La reacción de pNPP puede detenerse con NaOH 3 N y leerse a 405 nm.

Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).

The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.

Cantidad

Contains 40 mg substrate per tablet

Nota de preparación

Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl₂ and 1 mM ZnCl₂ or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl₂. Typically, a pNPP concentration of 1 mg/mL is used.

Otras notas

Sustrato para la determinación de fosfatasas ácidas y alcalinas.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)