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N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Sinónimos:

4-Nitrophenyl phosphate disodium salt solution, pNPP

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About This Item

Fórmula lineal:
O2NC6H4OP(O)(ONa)2
Número de CAS:
4264-83-9
Peso molecular:
263.05
Número MDL:
MFCD00007319
Código UNSPSC:
12352204
ID de la sustancia en PubChem:
24897835
NACRES:
NA.83

Nombre del producto

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

Formulario

liquid

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

cadena SMILES

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

Clave InChI

VIYFPAMJCJLZKD-UHFFFAOYSA-L

Descripción general

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Aplicación

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Acciones bioquímicas o fisiológicas

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Precaución

Stable at least one year when stored below 0 °C.

Forma física

Ready-to-use.

Pictogramas

Health hazardCorrosion
GHS08,GHS05

Palabra de señalización

Danger

Clasificaciones de peligro

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Órganos de actuación

Kidney,Liver,Blood

Código de clase de almacenamiento

8B - Non-combustible corrosive hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLCN6783
SLCL1721
SLCF2308
SLBP4121V
SLCB1535

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Scientific reports, 7(1), 17545-17545 (2017-12-14)
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