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Merck

P5306

Sigma-Aldrich

Papaína from papaya latex

lyophilized powder, aseptically filled

Sinónimos:

Papinasa

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

papaya (latex)

Nivel de calidad

esterilidad

aseptically filled

Formulario

lyophilized powder

actividad específica

≥8.0 units/mg protein

mol peso

23.4 kDa

solubilidad

H2O: soluble 1.2 mg/mL

temp. de almacenamiento

−20°C

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Descripción general

Papain is a thermostable enzyme and becomes inactive at 55°C. It has two domains and the catalytic region is present at the domain interface. The cysteine 25 in the active site performs the proteolysis by nucleophilic attack on the carbonyl carbon of a peptides. Papain has wide industrial applications in food and pharmaceutical industry.

Aplicación

Utilizada para producir fragmentos Fab de los anticuerpos. Se utiliza también para la disociación celular, ya que se ha demostrado que es más eficaz y menos lesiva con ciertos tejidos.
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies . It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P5306 is provided as a lyophilized powder that is aseptically filled. It has been used to digest mouse retinas to study the RAGE axis in nonproliferative diabetic retinopathy (NPDR).
The product from Sigma has been used in the digestion of extracellular matrix (ECM). It has also been used with other proteolytic enzymes for in situ protein degradation to produce crystals suitable for structure determination.

Acciones bioquímicas o fisiológicas

Una proteasa de cisteína que rompe los enlaces peptídicos de los aminoácidos básicos, la leucina o la glicina
pH óptimo 6,0-7,0
También hidroliza ésteres y amidas.
Papain papaya latex has antifungal activity against C. albicans. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Definición de unidad

One unit will hydrolyze 1.0 μmole of BAEE per minute at pH 6.2 at 25 deg C.

Nota de preparación

Prepared from P 4762

Otras notas

Véase más información sobre la papaína en www.sigma-aldrich.com/enzymeexplorer.

Inhibidor

Referencia del producto
Descripción
Precios

sustrato

Referencia del producto
Descripción
Precios

Pictogramas

Health hazardExclamation mark

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Gaetano R Barile et al.
Investigative ophthalmology & visual science, 46(8), 2916-2924 (2005-07-27)
The receptor for advanced glycation end products (AGEs) has been implicated in the pathogenesis of diabetic complications. This study was conducted to characterize the role of the RAGE axis in a murine model of nonproliferative diabetic retinopathy (NPDR). The retinas
Clara Malo et al.
Andrologia, 53(11), e14224-e14224 (2021-09-09)
This study evaluated the effects of freezing extenders, cryoprotectants and their concentrations, presence of Orvus Es Paste and freezing rates for cryopreserving dromedary camel sperm. Semen (five males; 2 ejaculates/male) was frozen in one of the following extenders (Green Buffer®
Improving thermostability of papain through structure-based protein engineering
Choudhury D, et al.
Protein engineering, design & selection : PEDS, 23(6), 457-467 (2010)
Amy Wernimont et al.
PloS one, 4(4), e5094-e5094 (2009-04-09)
For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, and among these only 13-15 crystallize in a form that enables structure determination. In 2007, Dong et al reported that the addition of trace amounts
M van Vijven et al.
Journal of the mechanical behavior of biomedical materials, 126, 105010-105010 (2021-12-14)
A ruptured anterior cruciate ligament (ACL) is often reconstructed with a multiple-strand autograft of a semitendinosus tendon alone or combined with a gracilis tendon. Up to 10% of patients experience graft rupture. This potentially results from excessive local tissue strains

Artículos

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

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