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Merck

N9876

Sigma-Aldrich

4-Nitrophenyl butyrate

lipase substrate, chromogenic, esterase substrate, ≥98%, liquid

Sinónimos:

p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester

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About This Item

Fórmula empírica (notación de Hill):
C10H11NO4
Número de CAS:
Peso molecular:
209.20
Beilstein/REAXYS Number:
1879729
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
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Nombre del producto

4-Nitrophenyl butyrate, ≥98%

Quality Level

assay

≥98%

form

liquid

density

1.19 g/mL at 20 °C (lit.)

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3

InChI key

DVDUMIQZEUTAGK-UHFFFAOYSA-N

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General description

4-Nitrophenyl butyrate is a substrate for esterase,[1] and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.[2]

Application

4-nitrophenyl butyrate has been used:
  • as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene[3]
  • as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity[4]
  • as a substrate to determine the effect of buffers and solvents on human carboxylesterase[5]

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Questions

  1. Good morning, I would like to know the concentration of the product N9876-1G, because in the container it says that there is 1 gram but the product is dissolved, so we do not know the concentration. Thank you

    1 answer
    1. This product is a neat or pure liquid with a density of 1.19 g/mL. The calculated molarity is 5.7M. The molarity is determined using the formula below:
      (density x volume x concentration )/(molecular weigh?)=molarity
      1.19g/mlx1000x1/209.20g/mol= 5.7M

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