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Merck

N3520

Sigma-Aldrich

Nutrient Mixture F-12 Ham

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Ham’s F-12

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.

Aplicación

Nutrient Mixture F-12 Ham is used for the following applications:
  • HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum[1]
  • Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)[2][3]
  • Used in media for the propagation of Cowdria ruminantium[4]
  • Used during preparation of primary mixed glial cultures[5]

Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.

Cantidad

Formulated to contain 11.1 grams of powder per liter of medium.

Reconstitución

Supplement with 2.5 g/L sodium bicarbonate.

Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Protocolos

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Questions

  1. Can you send me the full list of ingreadients of Ham's F-12 medium?

    1 answer
    1. The full list of components for this item can be found in the product data sheet. Please see the link below to review: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/224/n3520for.pdf

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