The full list of components for this item can be found in the product data sheet. Please see the link below to review: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/224/n3520for.pdf
N3520
Nutrient Mixture F-12 Ham
Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Sinónimos:
Ham’s F-12
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About This Item
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Formulario
powder
técnicas
cell culture | mammalian: suitable
componentes
NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
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Descripción general
The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.
Aplicación
Nutrient Mixture F-12 Ham is used for the following applications:
Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.
- HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum[1]
- Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)[2][3]
- Used in media for the propagation of Cowdria ruminantium[4]
- Used during preparation of primary mixed glial cultures[5]
Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.
Cantidad
Formulated to contain 11.1 grams of powder per liter of medium.
Reconstitución
Supplement with 2.5 g/L sodium bicarbonate.
suplemento
también adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Protocolos
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Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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