M0643

Medio esencial mínimo Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

• Sinónimos: MEM
• UNSPSC Code: 12352207
• NACRES: NA.75

Propiedades

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: HEPES: no; Earle’s salts (5% CO₂): yes; NaHCO₃: no; phenol red: yes; L-glutamine: yes; sodium pyruvate: no
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids.[1] It is widely used as a media formulation for many cell culture studies.[2] It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.[1]

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63[3], dispersed ventricular cells[4], and endometrial adenocarcinoma Ishikawa cells.[5]

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable