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N0252

Sigma-Aldrich

4-Nitrophenyl decanoate

lipase substrate, ≥98% (TLC), powder

Sinónimos:

4-Nitrophenyl caprate, Decanoic acid 4-nitrophenyl ester

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About This Item

Fórmula empírica (notación de Hill):
C16H23NO4
Número de CAS:
1956-09-8
Peso molecular:
293.36
Beilstein/REAXYS Number:
1915230
MDL number:
MFCD00057615
UNSPSC Code:
12352204
PubChem Substance ID:
329818409
NACRES:
NA.83

product name

4-Nitrophenyl decanoate, lipase substrate

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

chloroform: soluble 100 mg/mL, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C16H23NO4/c1-2-3-4-5-6-7-8-9-16(18)21-15-12-10-14(11-13-15)17(19)20/h10-13H,2-9H2,1H3

InChI key

RRNKCSIEGPOIKI-UHFFFAOYSA-N

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Application

4-Nitrophenyl decanoate has been used as a substrate for assessing the lipase activity spectrophotometrically. It has also been used as a substrate for estimating the carboxylesterases activity.
4-Nitrophenyl decanoate has been used as a substrate to assess the carboxylesterase production by recombinant Penicillium griseoroseum T55 strain. It has also been used for general esterase activity assay of the hepatopancreas extract.

Biochem/physiol Actions

4-Nitrophenyl decanoate is a substrate for lipase enzyme. The extracellular lipase has greater hydrolysis reaction velocity with 4-nitrophenyl decanoate when compared with other 4-nitropheny esters.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

pcodes

P280

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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V M Gumerov et al.
Prikladnaia biokhimiia i mikrobiologiia, 48(4), 376-382 (2012-10-06)
As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally
Methods in Molecular biology: Platelets and Megakaryocytes, volume 1, functional assays, 27-27 (2012)
Janaina Marques de Almeida et al.
Scientific reports, 8(1), 10000-10000 (2018-07-04)
We determined the effect of the His-tag on the structure, activity, stability and immobilization of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N-terminal His-tag and then removed the tag using tobacco etch virus
Isolation and characterization of an enzyme from the Greenshell? mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid
MacKenzie LA, et al.
Toxicon, 60(3), 406-419 (2012)
Dan Wu et al.
Frontiers in bioengineering and biotechnology, 8, 844-844 (2020-08-28)
The multiple inhibitors tolerance of microorganism is important in bioconversion of lignocellulosic biomass which is a promising renewable and sustainable source for biofuels and other chemicals. The disruption of an unknown α/β hydrolase, which was termed KmYME and located in
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