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Merck

MAK142

Sigma-Aldrich

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (Deep red)

Sinónimos:

Intracellular ROS Fluorescence Assay

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 200 fluorometric tests (Deep red)

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

neurological disorders; cancer

storage temp.

−20°C

General description

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.

Application


  • Diethyl Blechnic Exhibits Anti-Inflammatory and Antioxidative Activity via the TLR4/MyD88 Signaling Pathway in LPS-Stimulated RAW264.7 Cells.: This article uses fluorometric methods to explore the anti-inflammatory and antioxidative properties of diethyl blechnic, illustrating the role of ROS assays in elucidating the molecular mechanisms underlying the therapeutic effects of natural compounds (Yang S et al., 2019).

Suitability

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.

Principle

The Fluorometric Intracellular ROS Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells after 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and with detection at λex = 650/λem = 675 nm.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Bovine herpesviruses induce different cell death forms in neuronal and glial-derived tumor cell cultures.
Cardoso T C, et al.
Journal of Neurovirology, 22(6), 725-735 (2016)
The role of reactive oxygen species in the pathogenesis of Alzheimer?s disease, Parkinson?s disease, and Huntington?s disease: a mini review.
Manoharan S, et al.
Oxidative Medicine and Cellular Longevity, 2016 (2016)
Mitochondrial bioenergy alterations in avian HD11 macrophages infected with infectious bronchitis virus.
da Silva S E L, et al.
Archives of Virology, 1-7 (2018)
Reactive oxygen species: Re-evaluation of generation, monitoring and role in stress-signaling in phototrophic organisms.
Schmitt F J, et al.
Biochimica et Biophysica Acta - Bioenergetics, 1837(6), 835-848 (2014)
Jian-An Pan et al.
Redox biology, 46, 102120-102120 (2021-09-04)
The dose-dependent toxicity to cardiomyocytes has been well recognized as a central characteristic of doxorubicin (DOX)-induced cardiotoxicity (DIC), however, the pathogenesis of DIC in the cardiac microenvironment remains elusive. Irisin is a new hormone-like myokine released into the circulation in

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