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CS0790

Sigma-Aldrich

Antioxidant Assay Kit

sufficient for 200 tests

Sinónimos:

Oxidative Stress Detection Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
En este momento no podemos mostrarle ni los precios ni la disponibilidad

usage

sufficient for 200 tests

Quality Level

200

detection method

colorimetric

shipped in

wet ice

storage temp.

2-8°C

General description

Free radicals or reactive oxygen species (ROS) are produced during biochemical redox reactions as part of normal physiological cell metabolism (protection from infectious organisms) and as a response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation. Once formed, ROS attack cellular components causing damage to lipids, proteins, and DNA, which can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.
In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant.

Application

Antioxidant assay kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates.[1] It has also been used to measure the radical cation (2,2′-azino-di-[3-ethylbenzthiazoline sulphonate]) (ABTS+) scavenging capacity.[2][3]
It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva.

Suitability

Suitable for the measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates

Principle

The principle of the antioxidant assay is formation of a ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation, ABTS·+ , a soluble chromogen that is green in color and can be determined spectrophotometrically at 405 nm.

Preparation Note

Reagents are to be prepared with ultrapure water.

Reconstitution

Myoglobin Solutions are to be stored at -20C after reconstitution.
Trolox Working Solution is to be stored at -20C after reconstitution.
ABTS Substrate Solution is to be stored at 4C for 2 weeks or at -20C for 1 month.

Legal Information

Solo componentes del kit

Referencia del producto
Descripción
  • Assay Buffer 10X 30 mL
  • Stop Solution 20 mL
  • Hydrogen Peroxide, 3% solution 1 mL
  • Myoglobin from horse heart 2 x 1
  • (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1
  • ABTS, 10 mg tablets 4 tab
  • Phosphate-Citrate Buffer, pH 5 4 tab

related product

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000370913
0000370913
0000236832
0000236832
0000188799

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Questions

1–6 of 6 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can CS0790 be used for antioxidant capacity assessment in colored media without interference in the colorimetric assay?

    1 answer

    1. The kit functions by measuring antioxidant activity through the inhibition of ABTC oxidation. Oxidation of ABTC results in a green color reaction, which is reduced in the presence of antioxidants. Myoglobin is necessary for the oxidation of ABTC as it serves as a source of a radical cation. The types of samples suitable for use with this kit are detailed in the kit's bulletin, but it should be noted that bacterial culture media and oils are not included among them.

      Helpful?

  4. how is this test performed? can it use a HACH instrument? can this test identify BHT Butylated Hydroxy Toluene, 400-1000 ppm.

    1 answer

    1. Please see the kit's usage instructions posted here:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/281/332/cs0790bul.pdf

      The kit should be able to detect the antioxidant capacity of BHT (Butylated Hydroxy Toluene).

      If the HACH instrument is capable of reading absorbances in a 96-well plate at the required wavelength of 405 nm, it should be suitable for use with the kit.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is the pH of kit component A3605 in the Antioxidant Assay Kit, product CS0790?

    1 answer

    1. The pH of kit component A3605 in the Antioxidant Assay Kit, product CS0790, is approximately 5,  at 25°C. The adequacy/suitability of the pH of the assay buffer is assessed via the assay itself, and is tested for each lot of kits.

      Helpful?

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