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Merck
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Key Documents

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M1255

Sigma-Aldrich

Calcein Blue

Powder

Sinónimos:

4-Methylumbelliferone-8-methyliminodiacetic acid

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About This Item

Fórmula empírica (notación de Hill):
C15H15NO7
Número de CAS:
54375-47-2
Peso molecular:
321.28
EC Number:
259-129-8
MDL number:
MFCD00006867
UNSPSC Code:
12171500
PubChem Substance ID:
24896629
NACRES:
NA.47

product name

Calcein Blue, fluorescent dye

Quality Level

200

form

powder

technique(s)

titration: suitable

color

white to light yellow

solubility

1 M NaOH: 50 mg/mL

fluorescence

λex 322 nm; λem 445 nm in methanol

suitability

suitable for indicator for EDTA titration

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12

InChI

1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)

InChI key

RJPSHDMGSVVHFA-UHFFFAOYSA-N

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General description

Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.

Application

Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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The Journal of cell biology, 176(2), 231-241 (2007-01-11)
Neutrophil extracellular traps (NETs) are extracellular structures composed of chromatin and granule proteins that bind and kill microorganisms. We show that upon stimulation, the nuclei of neutrophils lose their shape, and the eu- and heterochromatin homogenize. Later, the nuclear envelope
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Nature communications, 10(1), 3731-3731 (2019-08-21)
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