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04511

Supelco

Live/Dead Cell Double Staining Kit

suitable for fluorescence

Sinónimos:

Staining kit for live/dead cells

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

suitability

suitable for fluorescence

Quality Level

100

storage temp.

−20°C

Application

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

Solo componentes del kit

Referencia del producto
Descripción
  • Solution A (Calcein AM solution) 4 × 50
  • Solution B (propidium iodide solution) 300 μL

related product

Referencia del producto
Descripción
Precios

92210

Timestrip Plus™ 0 °C

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

185.0 °F - closed cup

flash_point_c

85 °C - closed cup

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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