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M0275

Sigma-Aldrich

Medio esencial mínimo Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Sinónimos:

MEM

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

Nombre del producto

Medio esencial mínimo Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Nivel de calidad

400

esterilidad

sterile-filtered

Formulario

liquid

concentración

10 ×

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Aplicación

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.

Reconstitución

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

suplemento

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Descripción
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también adquirido normalmente con este producto

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Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBL8856
RNBL4490
RNBL2068
RNBK3740
RNBJ6472

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A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine
Drzewiecki KE, et al.
Technology, 5(04), 185-195 (2017)
Diffusion tensor imaging of articular cartilage as a measure of tissue microstructure
Meder R, et al.
Osteoarthritis and Cartilage, 14(9), 875-881 (2006)
Karl Norris et al.
Micromachines, 10(1) (2019-01-24)
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial
Zhu Cheng et al.
Science advances, 6(15), eaay0076-eaay0076 (2020-04-18)
Silicones are commonly used for lubrication of syringes, encapsulation of medical devices, and fabrication of surgical implants. While silicones are generally viewed as relatively inert to the cellular milieu, they can mediate a variety of inflammatory responses and other deleterious
Marie Nguyen et al.
Cell reports, 25(13), 3884-3893 (2018-12-28)
A major challenge in cancer research is the complexity of the tumor microenvironment, which includes the host immunological setting. Inspired by the emerging technology of organ-on-chip, we achieved 3D co-cultures in microfluidic devices (integrating four cell populations: cancer, immune, endothelial, and
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Artículos

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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