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L9017

Sigma-Aldrich

Lectina de Phaseolus vulgaris (frijol rojo)

Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder

Sinónimos:

Aglutinina de Phaseolus vulgaris, PHA

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About This Item

EC Number:
232-718-7
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Phaseolus vulgaris

Quality Level

300

product line

BioXtra

form

lyophilized powder

potency

≤17 μg per mL agglutination activity

purified by

affinity chromatography

impurities

salt, essentially free

storage temp.

2-8°C

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General description

Lectins are carbohydrate-binding proteins, that contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for phytohemagglutinin (PHA) swelling assay
  • for lymphocyte activation assay
  • to evaluate cell-mediated immune response

Biochem/physiol Actions

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It has anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin is associated with toxic allergens and hemagglutinins. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.
La PHA consta de dos especies moleculares, la eritroaglutinina (PHA-E), que tiene poca actividad mitógena y elevada actividad de eritroaglutinina, y la leoucoaglutinina (PHA-L), que tiene elevada actividad mitógena y leoucoaglutinante, pero muy baja actividad eritroaglutininante
La PHA-E no es específica de grupo sanguíneo, pero la aglutinación puede ser inhibida por ciertos oligosacáridos. La PHA-P es la forma proteica de la PHA antes de la separación y la purificación de la eritroaglutinina y la leoucoaglutinina. La PHA-M es la forma mucoproteínica. Los conjugados se preparan a partir de las correspondientes lectinas purificadas.

Quality

Mitogenic activity at ≤10 μg per mL.

Analysis Note

Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Chickadees faced with unpredictable food increase fat reserves but certain components of their immune function decline
Cornelius EA, et al.
Physiological and Biochemical Zoology : Pbz, 90(2), 190-200 (2017)
Diane L Bolton et al.
PLoS pathogens, 13(6), e1006445-e1006445 (2017-06-28)
CD4 T cells harboring HIV-1/SIV represent a formidable hurdle to eradicating infection, and yet their detailed phenotype remains unknown. Here we integrate two single-cell technologies, flow cytometry and highly multiplexed quantitative RT-PCR, to characterize SIV-infected CD4 T cells directly ex
Jon S Beadell et al.
PloS one, 2(9), e896-e896 (2007-09-20)
Dramatic declines of native Hawaiian avifauna due to the human-mediated emergence of avian malaria and pox prompted an examination of whether island taxa share a common altered immunological signature, potentially driven by reduced genetic diversity and reduced exposure to parasites.
Phaseolus vulgaris leucoagglutinin (PHA-L): A neuroanatomical tracer for electron microscopic analysis of synaptic circuitry in the cat's dorsal lateral geniculate nucleus
Cucchiaro JB and UhlrichDJ
Journal of Electron Microscopy Technique, 15(4), 352-368 (1990)
Aniuska Becerra-Artiles et al.
Cell reports, 39(11), 110952-110952 (2022-06-09)
Sequence homology between SARS-CoV-2 and common-cold human coronaviruses (HCoVs) raises the possibility that memory responses to prior HCoV infection can affect T cell response in COVID-19. We studied T cell responses to SARS-CoV-2 and HCoVs in convalescent COVID-19 donors and identified a
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Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

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