11082132001
Roche
Phytohemagglutinin-M (PHA-M)
from Phaseolus vulgaris
Sinónimos:
PHA-M
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About This Item
Código UNSPSC:
23201100
Productos recomendados
Nivel de calidad
esterilidad
non-sterile; 0.2 μm filtered
Formulario
lyophilized
envase
pkg of 20 mg
fabricante / nombre comercial
Roche
aplicaciones
hematology
temp. de almacenamiento
2-8°C
Descripción general
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.
Aplicación
Phytohemagglutinin (PHA) is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures.
Phytohemagglutinin-M (PHA-M) has been used in the activation of Jurkat and primary cells. It has also been used to treat polymorphonuclear blood cells(PMBCs)
Acciones bioquímicas o fisiológicas
Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.
Especificaciones
Biological activity: <10 μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes.
Nota de preparación
Working concentration: 2 - 10 μg/ml
Approximately 2 - 10 μg/ml for the stimulation of peripheral blood lymphocytes
Storage conditions (working solution): The reconstituted solution is stable for 2 weeks at 2 to 8 °C or for several months when stored in aliquots at -15 to -25 °C.
Approximately 2 - 10 μg/ml for the stimulation of peripheral blood lymphocytes
Storage conditions (working solution): The reconstituted solution is stable for 2 weeks at 2 to 8 °C or for several months when stored in aliquots at -15 to -25 °C.
Reconstitución
PHA-M should be reconstituted in sterile double-dist. water (final concentration: 2–10 mg/ml). Further dilution with medium or PBS (phosphate buffered saline).
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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