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Merck

L4895

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

FITC conjugate, lyophilized powder

Sinónimos:

WGA, Wheat germ agglutinin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

conjugate

FITC conjugate

Quality Level

form

lyophilized powder

potency

<50 μg/mL agglutination activity

composition

Protein, ~50% Lowry

extent of labeling

~2 mol FITC per mol protein

storage temp.

2-8°C

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General description

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.[1]

Application

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Lectin from Triticum vulgaris (wheat) has been used:
  • to treat transfected cells for immunofluorescence[2]
  • to stereotaxically inject into mice for neuronal tracing[3]
  • in immunohistochemistry for the analysis of myocytes[4]

Biochem/physiol Actions

Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.[1]
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains phosphate buffer salts

Preparation Note

Conjugates are prepared from affinity purified lectin.

related product

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Artículos

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

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Questions

1–2 of 2 Questions  
  1. Please is this product use in staining Arbuscular mycorrhizal fungi in root, for confocal microscopy examination?

    1 answer
    1. This product has not been tested specifically for use in fungal staining in plant tissue. However, FITC-labeled wheat germ agglutinin or lectin has been cited in the literature for such applications. Please see the link below to review one such publication:
      https://iris.unito.it/bitstream/2318/1740844/1/Carotenuto%20et%20al%202.pdf

      Helpful?

  2. Does Sigma provide a stain to visualize cell borders for human Cardiomyocytes derived from the GM23338 iPSC cell line?

    1 answer
    1. There is no recommended stain for cells from this type of cell line. However, for visualizing typical cardiomyocyte cell borders, one may consider L4895, as referenced by Vanhoutte D et al, Nat Commun. 2021 Jun 24;12(1):3928. L4895 is Sigma-Aldrich Lectin from Triticum vulgaris (wheat). The article titled "Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy" in Nat Commun. 2021 Jun 24;12(1):3928 may serve as a point of reference.

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