L5391
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
Isolectin B4 (BSI-B4), peroxidase conjugate, lyophilized powder
Sinónimos:
Bandeirea simplicifolia agglutinin, BS-I
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Productos recomendados
biological source
Bandieraea simplicifolia
Quality Level
conjugate
peroxidase conjugate
assay
≥90% protein basis (Warburg-Christian)
form
lyophilized powder
potency
<200 μg per mL agglutination activity (using human blood group B erythrocytes)
peroxidase activity
40-160 units/mg protein
composition
Protein, ~95% modified Warburg-Christian
technique(s)
blood typing: suitable
fractionation: suitable
storage temp.
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Application
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Other Notes
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Unit Definition
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Physical form
Contains sodium citrate buffer salts and calcium chloride
Preparation Note
Prepared from peroxidase type VI using a modification of the method of O′Sullivan, et al. Repurified by affinity chromatography after conjugation.
Analysis Note
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Hamed Zaer et al.
Scientific reports, 10(1), 16223-16223 (2020-10-03)
Stereotactic radiosurgery (SRS) has proven an effective tool for the treatment of brain tumors, arteriovenous malformation, and functional conditions. However, radiation-induced therapeutic effect in viable cells in functional SRS is also suggested. Evaluation of the proposed modulatory effect of irradiation
Christophe Dubois et al.
Journal of the American College of Cardiology, 55(20), 2232-2243 (2010-05-15)
We compared biological repair after acute myocardial infarction (AMI) with selected porcine progenitor cell populations. Cell types and mechanisms responsible for myocardial repair after AMI remain uncertain. In a blinded, randomized study, we infused autologous late-outgrowth endothelial progenitor cells (EPC)
Hélène Fe Gleitz et al.
EMBO molecular medicine, 10(7) (2018-06-10)
The pediatric lysosomal storage disorder mucopolysaccharidosis type II is caused by mutations in IDS, resulting in accumulation of heparan and dermatan sulfate, causing severe neurodegeneration, skeletal disease, and cardiorespiratory disease. Most patients manifest with cognitive symptoms, which cannot be treated
Dariusz Orlowski et al.
Neuromodulation : journal of the International Neuromodulation Society, 20(5), 417-423 (2017-02-22)
The use of Deep Brain Stimulation (DBS) in treatment of various brain disorders is constantly growing; however, the number of studies of the reaction of the brain tissue toward implanted leads is still limited. Therefore, the aim of our study
Luís Fernándes de Castro et al.
Tissue engineering. Part A, 16(4), 1157-1168 (2009-10-29)
The parathyroid-hormone-related protein (PTHrP) is an important modulator of bone formation and bone remodeling. High and/or prolonged glucocorticoid (GC) treatments inhibit PTHrP expression in osteoblastic cells and bone formation and repair. We assessed the ability of the N- and C-terminal
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico