L1882

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

• Sinónimos: WGA, Wheat germ agglutinin
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• conjugado: agarose conjugate
• Nivel de calidad: 200
• Formulario: saline suspension
• Extensión del etiquetado: 5-10 mg per mL
• Matriz: cross-linked 4% beaded agarose
• activación de la matriz: cyanogen bromide
• capacidad: ≥0.1 mg/mL binding capacity (ovomucoid)
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Aplicación

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Acciones bioquímicas o fisiológicas

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Forma física

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Nota de preparación

Conjugates are prepared from affinity purified lectin.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: Eyeshields, Gloves