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Merck

I4510

Sigma-Aldrich

Iminodiacetic acid Sepharose

aqueous ethanol suspension

Sinónimos:

Iminodiacetic acid Agarose, IDA Resin

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About This Item

MDL number:
UNSPSC Code:
23151817
PubChem Substance ID:
NACRES:
NA.56

form

aqueous ethanol suspension

Quality Level

matrix

Sepharose 6B Fast Flow

matrix activation

epoxy

matrix attachment

amino

matrix spacer

7 atoms

capacity

22-30 μmol/mL binding capacity (Zn2+)

storage temp.

2-8°C

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General description

I4510-50ML′s updated product number is GE17-0575-01
Iminodiacetic acid Sepharose is a chelating resin used for the purification of proteins.

Application

Iminodiacetic acid Sepharose has been slurry-packed into a tared polypropylene column for immobilized metal-ion affinity chromatography (IMAC) and for the preparation of the chelating sepharose column. It is an important component of metal affinity chromatography which immobilizes transition metal ions.
Iminodiacetic acid Sepharose is used is affinity chromatography, protein chromatography and chelating resins. Iminodiacetic acid Sepharose has been used to report a new procedure for the purification of two human monoclonal anti-HIV (human immunodeficiency virus) antibodies (mAbs 2G12 and 4E10).

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

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pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

96.8 °F

flash_point_c

36 °C


Certificados de análisis (COA)

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Purification of native proteins from the cytoplasm and periplasm of Escherichia coli using IMAC and histidine tails: a comparison of proteins and protocols
Lindner P, et al.
Methods, 4(1), 41-56 (1992)
Identification and quantification of histidine-rich glycoprotein (HRG) in the blood plasma of six marine bivalves
Abebe AT, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 147(1) (2007)
Isolation of isoelectric species of phosphorylated rhodopsin.
J H McDowell et al.
Methods in enzymology, 315, 70-76 (2000-03-29)
Formation of anhydrotetracycline during a high-temperature treatment of animal-derived feed contaminated with tetracycline.
Kuhne, M., et al.
Food Chemistry, 75(4), 423-429 (2001)
B Sutter et al.
Protein expression and purification, 19(1), 53-56 (2000-06-02)
The superoxide dismutases (EC 1.15.1.1) are a family of enzymes that catalyze the dismutation of superoxide radical anion to dioxygen and hydrogen peroxide. The active site contains a critical metal ion such as manganese, iron, or copper. The copper-containing protein

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