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H5505

Sigma-Aldrich

Histone from calf thymus

Type III-S

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About This Item

Número de CAS:
9064-47-5
Beilstein/REAXYS Number:
1895465
MDL number:
MFCD00131329
UNSPSC Code:
41106305
NACRES:
NA.51

biological source

bovine (calf) thymus

Quality Level

200

type

Type III-S

form

powder

concentration

≥60% protein (biuret)

technique(s)

activity assay: suitable

solubility

H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow

storage temp.

2-8°C

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Application

Histone is suitable for use:
  • as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
  • in Cdk5 activity assay
  • in histone H1 kinase assay
  • as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
  • as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells

Biochem/physiol Actions

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.

Features and Benefits

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Other Notes

Characterized as mainly subgroup f1 by SDS-PAGE

Preparation Note

Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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