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Merck

H0137

Sigma-Aldrich

HT Media Supplement (50×) Hybri-Max

lyophilized powder, suitable for hybridoma, BioXtra

Sinónimos:

HT Supplement, Hypoxanthine-Thymidin (HT) media supplement

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nombre del producto

HT Media Supplement (50×) Hybri-Max, lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma

Nivel de calidad

esterilidad

γ-irradiated

Línea del producto

BioXtra

Formulario

lyophilized powder

técnicas

cell culture | hybridoma: suitable

impurezas

endotoxin, tested

temp. de almacenamiento

−20°C

Aplicación

HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.

Reconstitución

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.

Información legal

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Simulated Cholinergic Reinnervation of beta (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing beta Cell and Cholinergic Cell
Jiao A, et al.
International Journal of Endocrinology, 2018 (2018)
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