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Key Documents

GE17-5073-01

SP Sepharose™ XL

Cytiva 17-5073-01, pack of 300 mL

Sinónimos:

Cation Exchange Resin, SP Sepharose XL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 300 mL

manufacturer/tradename

Cytiva 17-5073-01

matrix

6% cross-linked agarose with dextran surface extenders

particle size

45-165 μm

average diameter

90 μm

cleaning in place

3-14

working range

4-13

suitability

suitable for bioprocess medium

Categorías relacionadas

General description

SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.

Application

SP Sepharose™ XL is based on a highly cross-linked, bead formed 6% Agarose matrix similar to the well established SP Sepharose™ Fast FLow media. Dextran chains are covalently coupled to the Agarose matrix and modified with sulphopropyl (SP) strong cation exchange groups through chemically stable ether bonds. The stable Agarose matrix and long, flexible chains of Dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates


With its high loading capacities and excellent physical and chemical stability, SP Sepharose™ XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.


As member of the BioProcess media range, SP Sepharose™ XL meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • High loading capacities give more productive capture from clarified feedstocks.
  • Strong cation exchanger designed for capture steps
  • High chemical stability for effective CIP/sanitization
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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