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Key Documents

GE17-0729-10

SP Sepharose™ Fast Flow

Cytiva 17-0729-10, pack of 25 mL

Sinónimos:

SP Sepharose Column, Sepharose Fast Flow

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-0729-10

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

3-14

working range

4-13

capacity

70 mg binding capacity(ribonuclease A/ml medium)

suitability

suitable for bioprocess medium

General description

SP Sepharose™ Fast FLow is a strong cation exchanger based on the well established Sepharose™ Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Application

SP Sepharose™ Fast FLow is part of the Sepharose™ Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with sulphopropyl (Q) strong cation exchange groups. SP Sepharose™ Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with SP Sepharose™ Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, SP Sepharose™ Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Well-proven strong cation exchanger developed for industrial downstream processes.
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Capto MMC

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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Protocolos

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico