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Key Documents

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DFF100

Sigma-Aldrich

DEAE–Sepharose™

Fast Flow

Sinónimos:

Diethylaminoethyl–Sepharose™

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About This Item

Número de CAS:
57407-08-6
MDL number:
MFCD00146630
UNSPSC Code:
47101511
NACRES:
NA.56

Quality Level

200

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

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Categorías relacionadas

General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose has been used in:
  • anion exchange chromatography
  • to screen polyisoprenyl phosphate phosphatase activity
  • to purify isoinhibitors
  • for the purification of human immunodeficiency virus (HIV)
  • glycoprotein envelope (gp140 env)

DEAE-Sepharose™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Referencia del producto
Descripción
Precios

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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G L Yewey et al.
Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)
Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal
Jessie Fernandez et al.
mBio, 12(1) (2021-02-11)
Rice blast disease caused by Magnaporthe oryzae is a devastating disease of cultivated rice worldwide. Infections by this fungus lead to a significant reduction in rice yields and threats to food security. To gain better insight into growth and cell
T M Schmidt et al.
Applied and environmental microbiology, 55(10), 2607-2612 (1989-10-01)
Luminescence of batch cultures of Xenorhabdus luminescens was maximal when cultures approached stationary phase; the onset of in vivo luminescence coincided with a burst of synthesis of bacterial luciferase, the enzyme responsible for luminescence. Expression of luciferase was aldehyde limited
Advances in Potato Chemistry and Technology (2016)
Growth cone collapse and neurite retraction from cultured <I>Helisoma</I> neurons in response to antibody Fab fragments against an extracellular matrix protein.
Miller, J.D., et al.
Dev. Brain Res., 79(2), 203-218 (1994)
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