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GE17-0404-01

HiTrap® Protein G High Performance

Cytiva 17-0404-01, pack of 5 × 1 mL

Sinónimos:

Protein purification column

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

Formulario

prepacked column

reticulante

cross-linked

envase

pack of 5 × 1 mL

fabricante / nombre comercial

Cytiva 17-0404-01

condiciones de almacenamiento

(20% Ehtanol)
 (20% Ethanol)

Parámetros

<4 mL/min flow rate
42 psi

técnicas

liquid chromatography (LC): suitable

lecho tamaño

7 mm × 25 mm

lecho volumen

1 mL

columna D.I.

7 mm

tamaño

Matriz

(cross-linked agarose, spherical)

grupo activo de la matriz

Protein G functional group

tam. prom. pieza

34 μm

cleaning

2-9

Intervalo de trabajo

3-9

capacidad

>1 mg binding capacity (lgG/column)
 25 mg binding capacity(human IgG/column)

técnica de separación

affinity

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Características y beneficios

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Protocolos

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This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

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