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GE17-5158-01

HiTrap® Q XL

Cytiva 17-5158-01, pack of 5 × 1 mL

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

quaternary amine

descripción

Ion Exchanger Type (value)

Formulario

prepacked column

reticulante

cross-linked

envase

pack of 5 × 1 mL

fabricante / nombre comercial

Cytiva 17-5158-01

condiciones de almacenamiento

(20% Ethanol)

Parámetros

-70 psi max. pressure (max flow rate = 20 ml/min)

técnicas

liquid chromatography (LC): suitable

lecho tamaño

7 mm × 25 mm

lecho volumen

1 mL

columna D.I.

7 mm

Matriz

6% cross-linked agarose with dextran surface extenders

grupo activo de la matriz

Q functional group, ammonium group

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning

2-14

Intervalo de trabajo

2-12

capacidad

binding capacity(>= 160 mg albumin / mL)
 ion exchange capacity(0.18-0.26 mmol Cl / mL)

idoneidad

suitable for bioprocess medium

Descripción general

HiTrap® Q XL are strong anion exchangers prepacked with Q Sepharose™ XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Aplicación

Q Sepharose™ XL and SP Sepharose™ XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

Características y beneficios

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from Cytiva.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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