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G2150

Sigma-Aldrich

L-Glutamina solution

200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma

Sinónimos:

L-Glutamine for cell culture

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About This Item

Número de CAS:
56-85-9
MDL number:
MFCD00008044
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24895088
NACRES:
NA.75

grade

Hybri-Max™

Quality Level

300

sterility

sterile-filtered

assay

≥99% (TLC)

form

solution

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

color

clear colorless

shipped in

dry ice

storage temp.

−20°C

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

rat ... Ggt1(116568)

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General description

La L-glutamina es un aminoácido esencial que es un componente crucial de los medios de cultivo donde actúa como una fuente importante de energía para las células en cultivo. La L-glutamina es muy estable en forma de polvo seco y de disolución congelada. En los medios líquidos o las disoluciones de reserva, sin embargo, la L-glutamina se degrada con relativa rapidez. El rendimiento celular óptimo normalmente requiere el enriquecimiento de los medios con L-glutamina antes de su uso.

Application

The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.

Preparation Note

Prepared in cell culture grade water.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Application

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related product

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Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Production of human antibodies from transgenic mice.
C Geoffrey Davis et al.
Methods in molecular biology (Clifton, N.J.), 248, 191-200 (2004-02-19)
Marina K Balabekova et al.
Drug and chemical toxicology, 44(3), 277-285 (2019-03-09)
Heavy metal pollution is rapidly increasing in the environment. It has been shown that exposure to vanadium and chromium is able to alter the immune response. Nevertheless, the mechanisms by which these metal pollutants mediate their immunomodulatory effects are not
Abraham L Moure et al.
Journal of medicinal chemistry, 63(9), 4732-4748 (2020-04-11)
Screening of a GSK-proprietary library against intracellular Mycobacterium tuberculosis identified 1, a thioalkylbenzoxazole hit. Biological profiling and mutant analysis revealed that this compound is a prodrug that is bioactivated by the mycobacterial enzyme MymA. A hit-expansion program including design, synthesis
Goonho Park et al.
Cell reports, 31(13), 107839-107839 (2020-07-02)
Amyloid β-protein (Aβ) toxicity is hypothesized to play a seminal role in Alzheimer's disease (AD) pathogenesis. However, it remains unclear how Aβ causes synaptic dysfunction and synapse loss. We hypothesize that one mechanism of Aβ-induced synaptic injury is related to
A U Lekmanov et al.
Anesteziologiia i reanimatologiia, (1)(1), 49-51 (2013-07-03)
Comparing analysis of randomized study was conducted. 40 Pediatric patients (2-15 years old) with heavy thermic burns and concomitant injuries were recruited into the study to assess the glutamine solution intravenous use efficiency. Parenteral feeding and feeding with glutamine solution
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Chromatograms

application for HPLC

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