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Merck

G1546

Sigma-Aldrich

Monoclonal Anti-Green Fluorescent Protein (GFP), C-terminal antibody produced in mouse

clone GSN149, purified from hybridoma cell culture

Sinónimos:

Mouse Anti-GFP

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

GSN149, monoclonal

Formulario

buffered aqueous solution

envase

antibody small pack of 25 μL

técnicas

indirect ELISA: suitable
western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Descripción general

Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the hybridoma GSN149 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of the Green fluorescent protein from jellyfish Aequorea victoria. GFP is a 27 kDa protein (238 amino acids) that can produce a strong green fluorescence without the need for a substrate. It absorbs light maximally at 395 nm and emits a bright green fluorescence with a peak at 509 nm. The GFP chromophore is formed through cyclization and oxidation of an internal tripeptide motif (Ser65, Gly69 and Tyr66).
The antibody reacts specifically with GFP-fusion proteins.

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse was used in surface plasmon resonance (SPR) gold chip analysis to study the cysteine- and domain-mediated immobilization of enhanced GFP model protein.

Acciones bioquímicas o fisiológicas

GFP is implicated in protein detection and localization in living cells, as well as gene expression monitoring in prokaryotes and eukaryotes. It detects protein-protein interactions in living cells.
Green fluorescent protein, produced in the jellyfish, Aequorea victoria, acts as secondary fluorescent protein in an energy transfer reaction to produce green light. It ability to produce a highly visible, internal fluorophore is widely applicable as marker for gene expression and to produce chimeric proteins to understand protein biochemistry.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Visual screening of microspore-derived transgenic barley (Hordeum vulgare L.) with green-fluorescent protein
Carlson AR, et al.
Plant Cell Reports, 20(4), 331-337 (2001)
Kyoungsook Park et al.
The Analyst, 136(12), 2506-2511 (2011-04-27)
Here we report an effective method for protein immobilization on a surface plasmon resonance (SPR) gold chip, describing the combination of cysteine- and oligomerization domain-mediated immobilization of enhanced green fluorescent protein (EGFP) as a model protein for the purpose of
The dynamic microbe: green fluorescent protein brings bacteria to light
Southward CM and Surette MG
Molecular Microbiology, 45(5), 1191-1196 (2002)
GFP: Lighting up life.
Martin Chalfie
Proceedings of the National Academy of Sciences of the United States of America, 106(25), 10073-10080 (2009-06-26)
Yuping Mei et al.
Nature communications, 6, 7316-7316 (2015-06-23)
PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small

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