G1546
Monoclonal Anti-Green Fluorescent Protein (GFP), C-terminal antibody produced in mouse
clone GSN149, purified from hybridoma cell culture
Sinónimos:
Mouse Anti-GFP
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.46
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified from hybridoma cell culture
tipo de anticuerpo
primary antibodies
clon
GSN149, monoclonal
Formulario
buffered aqueous solution
envase
antibody small pack of 25 μL
técnicas
indirect ELISA: suitable
western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins
isotipo
IgG1
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Descripción general
Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the hybridoma GSN149 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of the Green fluorescent protein from jellyfish Aequorea victoria. GFP is a 27 kDa protein (238 amino acids) that can produce a strong green fluorescence without the need for a substrate. It absorbs light maximally at 395 nm and emits a bright green fluorescence with a peak at 509 nm. The GFP chromophore is formed through cyclization and oxidation of an internal tripeptide motif (Ser65, Gly69 and Tyr66).
The antibody reacts specifically with GFP-fusion proteins.
Aplicación
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse was used in surface plasmon resonance (SPR) gold chip analysis to study the cysteine- and domain-mediated immobilization of enhanced GFP model protein.
Acciones bioquímicas o fisiológicas
GFP is implicated in protein detection and localization in living cells, as well as gene expression monitoring in prokaryotes and eukaryotes. It detects protein-protein interactions in living cells.
Green fluorescent protein, produced in the jellyfish, Aequorea victoria, acts as secondary fluorescent protein in an energy transfer reaction to produce green light. It ability to produce a highly visible, internal fluorophore is widely applicable as marker for gene expression and to produce chimeric proteins to understand protein biochemistry.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Los clientes también vieron
Kyoungsook Park et al.
The Analyst, 136(12), 2506-2511 (2011-04-27)
Here we report an effective method for protein immobilization on a surface plasmon resonance (SPR) gold chip, describing the combination of cysteine- and oligomerization domain-mediated immobilization of enhanced green fluorescent protein (EGFP) as a model protein for the purpose of
The dynamic microbe: green fluorescent protein brings bacteria to light
Southward CM and Surette MG
Molecular Microbiology, 45(5), 1191-1196 (2002)
GFP: Lighting up life.
Martin Chalfie
Proceedings of the National Academy of Sciences of the United States of America, 106(25), 10073-10080 (2009-06-26)
Visual screening of microspore-derived transgenic barley (Hordeum vulgare L.) with green-fluorescent protein
Carlson AR, et al.
Plant Cell Reports, 20(4), 331-337 (2001)
A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease.
Petra C Boevink et al.
Nature communications, 7, 10311-10311 (2016-01-30)
Plant pathogens deliver effectors to alter host processes. Knowledge of how effectors target and manipulate host proteins is critical to understand crop disease. Here, we show that in planta expression of the RXLR effector Pi04314 enhances leaf colonization by Phytophthora
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