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Merck

F4262

Sigma-Aldrich

Anti-Cat IgG (whole molecule)−FITC antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:

Goat Anti-Cat IgG (whole molecule)−Fluorescein isothiocyanate

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

conjugado

FITC conjugate

forma del anticuerpo

IgG fraction of antiserum

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

condiciones de almacenamiento

protect from light

técnicas

direct immunofluorescence: 1:32

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Descripción general

IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.

Especificidad

Anti-Cat IgG (whole molecule)-FITC antibody specifically recognizes cat IgG. Goat anti-cat IgG is conjugated to Fluorescein Isothiocyanate (FITC) in an alkaline reaction, then further purified to remove unbound FITC.

Inmunógeno

Purified cat IgG
Purified cat IgG.

Aplicación

Anti-Cat IgG (whole molecule)-FITC antibody has been used in immunofluorescence antibody test (IFAT), immunofluorescence.
Anti-Cat IgG (whole molecule)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:32. The antibody was used in indirect fluorescent antibody test (IFAT) to detect Toxoplasma specific antibodies in cat at a dilution of 1:100.
Anti-cat IgG (whole molecule) −FITC antibody can be used in direct (1:32) and indirect immunofluorescence (dilution 1:64).

Acciones bioquímicas o fisiológicas

IgG antibody plays a crucial role in humoral immune responses such as phagocytosis, complement activation, placental transport and cell surface-receptor binding. FITC (fluorescein isothiocyanate), a fluorochrome dye conjugation is useful in localization of antigen-antibody interaction of biological structures like cells or tissues. Anti-cat IgG (whole molecule) -FITC antibody can be used in immunofluorescence study of exfoliative dermatitis in cats. Goat anti-cat IgG antibody is immonospecific for cat IgG.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Gabriela Hartmann et al.
Parasitology research, 122(11), 2631-2639 (2023-09-05)
Feline leishmanial infection is reported worldwide, but the epidemiological role of domestic cats in the leishmaniasis cycle remains unclear, and cats might act as cryptic reservoir hosts in endemic areas with no feline leishmaniosis cases. Considering that, a serological screening
S Rottenberg et al.
Veterinary pathology, 41(4), 429-433 (2004-07-03)
Five cases of exfoliative dermatitis in cats were presented from 1996 to 2002 in which a feline thymoma was found by postmortem or postsurgical examination. Besides abundant exfoliation of keratin squames and layers, the histologic picture of the skin revealed
Maria Mercedes Nogueras et al.
PloS one, 8(8), e71386-e71386 (2013-08-14)
Rickettsiatyphi is the etiological agent of murine typhus (MT), a disease transmitted by two cycles: rat-flea-rat, and peridomestic cycle. Murine typhus is often misdiagnosed and underreported. A correct diagnosis is important because MT can cause severe illness and death. Our
Feline Leishmania infection in a canine leishmaniasis endemic region, Portugal
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Veterinary Parasitology, 174(3-4), 336-340 (2010)
Exploring the epidemiological role of the Eurasian lynx (Lynx lynx) in the life cycle of Toxoplasma gondii.
Scherrer, et al.
International Journal for Parasitology. Parasites and Wildlife, 21, 10-Jan-10-Jan (2023)

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