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A25120

Sigma-Aldrich

DEAE Sephadex®

Sinónimos:

DEAE – Sephadex® A-25 chloride form

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About This Item

Número de CAS:
12609-80-2
MDL number:
MFCD00132778
UNSPSC Code:
47101511
NACRES:
NA.56

form

powder

Quality Level

200

technique(s)

activity assay: suitable

matrix

cross-linked dextran

matrix active group

functional group

bead size

40-125 μm (dry)

pore size

~30,000 Da exclusion limit

operating pH

2-9

capacity

3-4 meq/g ion exchange capacity

compatibility

mode of use mixed weak and strong anion exchange chromatography

General description

The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.

A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02

Application

DEAE-Sephadex® A-25 has been used:
  • to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
  • in the estimation of GLS content in Chinese kale sprouts
  • production and purification of endozepine from astrocytes
  • to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients

Features and Benefits

  • Weak anion exchanger
  • Suitable for batch techniques
  • High binding capacities
  • Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

Legal Information

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

replaced by

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Scott N Boyle et al.
Biochemistry, 46(41), 11614-11620 (2007-09-26)
Abl family kinases have been implicated in the regulation of cell morphogenesis and migration, but the molecular mechanisms through which they operate are not fully elucidated. We applied the bump-hole technique, pioneered by Shokat and colleagues, to identify direct substrates
DEAE-silica gel and DEAE-controlled porous glass as ion exchangers for the isolation of glycolipids.
S K Kundu
Methods in enzymology, 72, 174-185 (1981-01-01)
William F Loomis et al.
The Journal of biological chemistry, 285(28), 21359-21365 (2010-05-11)
Acyl-CoA-binding protein (ACBP) functions both intracellularly as part of fatty acid metabolism and extracellularly as diazepam binding inhibitor, the precursor of endozepine peptides. Two of these peptides, ODN and TTN, bind to the GABA(A) receptor and modulate its sensitivity to
Dong-Lin Wang et al.
Journal of agricultural and food chemistry, 50(2), 262-265 (2002-01-10)
Among 10 lipases tested, Candida rugosa lipase exhibited the best ability to catalyze the resolution of dl-menthol in organic solvent. The lipase was immobilized on different carriers, and the experiment was carried out with different acyl donors. The high yield
M J Hollingsworth et al.
Molecular and cellular biology, 6(4), 1058-1064 (1986-04-01)
A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA.
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