D0550

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

• Sinónimos: 3,5-Dinitro-2-hydroxybenzoic acid, DNS
• Fórmula lineal: (O₂N)₂C₆H₂-2-(OH)CO₂H
• Número de CAS: 609-99-4
• Peso molecular: 228.12
• Beilstein/REAXYS Number: 2220661
• EC Number: 210-204-3
• MDL number: MFCD00007104
• UNSPSC Code: 12171500
• PubChem Substance ID: 24893243
• NACRES: NA.47

Propiedades

• Quality Level: 200
• assay: ≥98% (HPLC and titration)
• form: powder
• mp: 168-172 °C (lit.)
• solubility: H₂O: 50 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
• InChI key: LWFUFLREGJMOIZ-UHFFFAOYSA-N

Descripción

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Información de seguridad

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H318
• pcodes: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1
• Storage Class: 4.1A - Other explosive hazardous materials
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves