C8946

α-Chymotrypsin from human pancreas

lyophilized powder

• Número de CAS: 9004-07-3
• Comisión internacional de enzimas: 3.4.21.1 (BRENDA, IUBMB)
• EC Number: 232-671-2
• MDL number: MFCD01323069
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: human pancreas
• Quality Level: 200
• form: lyophilized powder
• mol wt: 25 kDa
• packaging: vial of ≥10 BTEE units
• UniProt accession no.: P17538
• storage temp.: −20°C
• Gene Information: human ... CTRB1(1504)

Descripción

General description

α-Chymotrypsin belongs to the trypsin family of serine proteases. It is secreted as an inactive precursor chymotrypsinogen to the intestine. The cleavage of peptide bond at amino acid 148 forms α-chymotrypsin. Chymotrypsinogen is encoded by the gene CTRB1 and is mapped to 16q23.1 in the human chromosome.

Application

α-Chymotrypsin from human pancreas has been used to test inhibition by N-alkyl isatins against mammalian serine proteases.

Human α-chymotrypsin has been used in a study to assess the quantitative structure-activity relationships for organophosphates binding to trypsin and chymotrypsin. Human α-chymotrypsin has also been used in a study to investigate the direct detection of native proteins in biological matrices using extractive electrospray ionization mass spectrometry.

Biochem/physiol Actions

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.

Unit Definition

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25°C.

Analysis Note

Protein determined by A1%/280

Información de seguridad

• pictograms: GHS08,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H315,H319,H334,H335,H400
• pcodes: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves